MGRC

GENOME SEQUENCING SERVICES

GENOME SEQUENCING SERVICES

Whole genome sequencing (WGS) is carried out to obtain the sequence of the entire genome from any eukaryotic or prokaryotic organism. De novo sequencing refers to the sequencing of a target organism without prior knowledge of its genome, while resequencing uses a reference sequence from a similar organism that has previously been sequenced to aid with the downstream analysis.

Whole genome sequencing can be used to sequence the genomes of a broad range of organisms, from the smallest virus right up to the largest plant. WGS can be used to identify alleles or variations in a genome. This method of sequencing can be applied to a variety of projects, including the following:

• Human and Mammalian Genetics

  Detection and identification of known and novel mutations using whole genome sequencing and resequencing

• Microbial Sequencing

  De novo sequencing and resequencing of multiple strains of microbes

• Agrigenomics

  De novo sequencing and resequencing of plant and crop genomes that are of commercial and research interest

• Pathogen Detection

  Detection and characterisation of foreign DNA from infected plants and animals, or from clinical samples

What We Do?

• Discuss your sequencing project in detail, and identify key expectations and targets
• Design a sequencing protocol optimised for your needs
• Provide guidance and consultation on sample preparation methods e.g. the quantity and quality of nucleic acid required
• Test the quality of received samples
• Prepare the nucleic acid for sequencing according to a protocol optimised for your project
• Sequence the sample using a next-generation sequencing platform best suited to your project
• Help manage your sequencing project and keep you updated on progress
• Deliver your data on a hard drive, or via download from a secure server

Additionally, we can provide bioinformatics services to suit your requirements, capabilities and budget. These services include analysis and screening of data quality, complete assembly of genomes, and annotation of genes.

How Do You Start?

1. Fill in the enquiry form or contact MGRC at enquiries@mgrc.com.my
2. Tell us about your project and what you would like to achieve.

• What organism would you like to sequence?
• Is this a de novo sequencing or resequencing project? If the latter, what reference sequence would you prefer?
• Do you know the approximate size of the genome?
• Do you have any information on its genomic content (GC content, repeat regions)?

3. We will contact you to discuss your requirements in greater detail.