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Eukaryotic Transcriptomics
Engine for Biological Discovery
Date : 15 – 16 April 2015
Time : 9 am – 5 pm
Venue :
MGRC Training Centre
37-5, Level 5
Boulevard Signature Office
Mid Valley City
59200 Kuala Lumpur
 
Fee : RM2,000 per person
 
Download workshop flyer here.
Workshop Online Registration
 
Course Outcomes
You will be able to:

  • identify the different classes of RNAs which are transcribed in eukaryotes
  • understand the activities of these RNAs and how to sequence them
  • use current bioinformatics tools for transcriptome assembly
  • annotate assembled transcripts
  • design differential expression experiments and identify differentially expressed genes / transcripts
  • upload completed transcriptomes to NCBI prior to publication
Requirements
You should have:

  • basic computer competency
  • strong understanding of biology
  • keen interest in bioinformatics and genomics
  • basic experience with UNIX operating system
Workshop Agenda
Day 1
Morning Session: An Overview of Eukaryotic Transcriptomics
  • Background to eukaryote transcriptomes
  • Structure of transcriptomes
  • mRNAs, lncRNAs, sRNAs and miRNAs
  • Transcriptome assembly
Afternoon Session: Hands-on Training
  • QC of reads
  • Transcriptome assembly using Trinity
  • Transcript annotation
  • Transcriptome assembly
Day 2
Morning Session: Technology Overview

  • Illumina
  • PacBio
  • Arrays
  • RNA-seq versus arrays
Afternoon Session I: Experimental Design (Hands-on)

  • Differential gene expression
  • Differential transcript expression
  • Isoforms
  • Annotation of transcripts
  • Finding orthology
  • Functional annotation
Afternoon Session II: Q & A and Feedback Session