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Applications |
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De novo sequencing, resequencing, transcriptome analysis, gene regulation studies, epigenetic changes, metagenomics & microbial diversity, paleogenomics analysis |
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Resequencing, transcriptome analysis, gene regulation, ChIP, De novo with paired-end for smaller genomes, epigenetic changes |
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Targeted resequencing, gene expression, microRNA discovery, ChIP, whole genome resequencing |
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Visualization |
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Light from chemical reaction |
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Color by fluorescence tags |
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Four fluorescence tags |
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Read length |
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200 to 300 bases |
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25 to 40 bases |
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35 bases |
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Sequence generated per run |
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More than 80 million bases |
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More than a billion bases |
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More than 3 billion bases |
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Run time |
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7.5 hours |
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3 days, 6 days for paired end |
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Fragment library: 4 days
Mate pairs library: 8 days |
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Number of samples |
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2, 4 or 13 samples per run |
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8 samples per run |
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1 to 16 samples per run |
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Single-read accuracy |
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99.5% |
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99.9% |
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97% |
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Types of error |
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Homopolymer errors, phase errors, chimaeras, duplicated fragments |
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Read quality declines towards end of read |
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Mis-incorporated bases |
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Multiplexing |
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Up to 16 samples/plate; bar-coding tags in development |
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8 samples/slide; bar-coding tags in development |
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4 samples/slide; bar-coding tags in development |
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Paired-end reads |
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Yes; read size 250bp; insert size variable |
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Yes; read size 35bp; insert size 250bp, 500bp contiguous fragments, 1 - 15kb pairs library pairings |
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Yes; read size 25bp; insert size 3kb - 8kb |
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